FIELD: biotechnology.
SUBSTANCE: group of inventions is proposed related to biotechnology – Streptomyces hygroscopicus VKPM Ac-2079 strain – astolides A and B antibiotics producer and a method for producing astolide A and astolide B antibiotics using the specified strain. Strain of Streptomyces hygroscopicus 18, which has antifungal activity against A. niger, C. albicans and C. tropicalis, is deposited in VKPM under the registration number VKPM Ac-2079. Method of obtaining A and B astolide antibiotics involves the cultivation of a strain of Streptomyces hygroscopicus VKPM Ac-2079 on soy nutrient medium at a temperature of 27 °C for 5–6 days. Obtained culture liquid is centrifuged, filtered and extracted with ethanol, followed by precipitation with ethyl acetate and isolation of A astolide and B astolide antibiotics using high performance liquid chromatography using a linear gradient of acetonitrile in water.
EFFECT: group of inventions allows to obtain new antifungal A and B astolide antibiotics, which have antifungal activity against Aspergillus niger, Candida albicans and Candida tropicalis.
2 cl, 5 dwg, 5 tbl, 5 ex
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