FIELD: biotechnology.
SUBSTANCE: described is an adeno-associated virus (AAV) vector for delivering a heterologous nucleic acid sequence to a subject, comprising a VP1 capsid protein, which contains one or more lysine substitutions, where one lysine replacement is K137R. Said vector additionally contains a minigene comprising AAV inverted terminal repeats and a heterologous nucleic acid sequence operably linked to regulatory sequences which direct expression of a product from the heterologous nucleic acid sequence in a host cell, said lysine substitution being effective to inhibit ubiquitination of said capsid protein. Application of the claimed vector is also described.
EFFECT: transduction of said AAV vector in the target cell increases.
29 cl, 9 tbl, 1 ex, 20 dwg
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