FIELD: biotechnology.
SUBSTANCE: immortalized porcine alveolar macrophage (PAM) is proposed. Said PAM is susceptible to porcine respiratory and reproductive virus (PRRSV), expresses an SV40 T-antigen, wherein a DNA comprising the SV40 T-antigen is inserted into the cellular genome using a transposon that does not comprises large terminal repeats (LTR) of retroviral DNA. Cell culture, a method for producing an immortalized PAM, a method for replicating PRRSV, and a method for producing a vaccine comprising PRRSV are also disclosed.
EFFECT: replication of PRRSV virus.
12 cl, 7 dwg, 1 ex
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