METHOD OF OBTAINING RECOMBINANT VESICULAR STOMATITIS VIRUS Russian patent published in 2023 - IPC C12N15/861 

FIELD: biotechnology.

SUBSTANCE: method of producing recombinant vesicular stomatitis virus is described. Cells stably expressing the DNA-dependent RNA polymerase of bacteriophage T7 are transfected using a mixture of plasmid expression vectors and polyethylenimine in a DNA: polyethylenimine weight ratio of 5:1. The mentioned mixture contains four plasmid expression vectors pCAG-VSVN, pCAG-VSVP, pCAG-VSVL and pCAG-VSVG, each of which encodes one of the vesicular stomatitis virus proteins N, P, L, G, respectively, under the control of the CAG promoter, and a plasmid an expression vector encoding the antigenomic sense RNA of the vesicular stomatitis virus genome under the control of the T7 promoter, in a ratio of 3:5:1:4:8 or 3:5:1:8:10. The mixture is cultivated, the culture liquid is collected, other cells are transduced with this liquid, then the cells are cultivated, and the conditioned culture liquid is collected.

EFFECT: method of obtaining recombinant vesicular stomatitis virus.

18 cl, 17 dwg, 9 ex