FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. Disclosed is an immunogenic composition comprising (I) a primer component consisting of recombinant pseudo adenoviral particles: particles carrying the gene tul4 Francisella tularensis with sequence SEQ ID NO 1, particles carrying the Francisella tularensis fopA gene with the sequence SEQ ID NO 3 and particles carrying the dnaK Francisella tularensis gene with the sequence SEQ ID NO 5, wherein said genes are optimized for expression in eukaryotic cells, and/or (II) a booster component comprising recombinant proteins: DBD-Tul4 protein with sequence SEQ ID NO 16, molecular weight 30.0 kDa containing dextrans-binding dextransuncrase domain of L. citreum KM20, presented by amino acid residues 1–140 of SEQ ID NO 16, Gly-Ser spacer, represented by residues 141–150 of SEQ ID NO 16, and Tul4 protein sequence of Francisella tularensis, represented by residues 151–282 of SEQ ID NO 16; 6-His-FopA protein with sequence SEQ ID NO 18, molecular weight 40.5 kDa; protein DnaK-6His with sequence SEQ ID NO 20, molecular weight 70.4 kDa, and also containing a pharmaceutically acceptable carrier or diluent and a method for its production.
EFFECT: invention provides a stable and stable immune response to Francisella tularensis.
5 cl, 21 dwg, 1 tbl, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA ENCODING TUL4spCBD RECOMBINANT PROTEIN SYNTHESIS, M15[pREP4, pTUL4spCBD] ESCHERICHIA COLI STRAIN AS PRODUCER OF TUL4spCBD RECOMBINANT PROTEIN, TUL4spCBD RECOMBINANT PROTEIN, METHOD FOR PRODUCTION THEREOF, AND METHOD FOR PRODUCTION OF SPECIFIC ANTIBODY AGAINST TUL4spCBD PROTEIN | 2004 |
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| METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON CFP10-DBD HYBRID PROTEIN AND DEXTRANE; pCFP10-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pCFP10-DBD] STRAIN; CFP10-DBD CHIMERIC PROTEIN AND THEIR APPLICATION | 2013 |
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