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IPC

C12N15/00(2006-01-01)

C12N1/21(2006-01-01)

(21) (22)

Application

2013119210/10, 2013-04-25

(24)

Start date

2013-04-25

(22)

Actual filing date

2013-04-25

(45)

Published

2014-06-20

(72)

Inventor

Tkachuk Artem PetrovichLjashchuk Aleksandr MikhajlovichAksenova Ekaterina IvanovnaPoletaeva Nina NikolaevnaGalushkina Zoja MikhajlovnaSergienko Ol'Ga Vasil'EvnaApt Aleksandr SolomonovichKarjagina-Zhulina Anna StanislavovnaLunin Vladimir GlebovichGintsburg Aleksandr Leonidovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Institut Ehpidemiologii I Mikrobiologii Imeni Pochetnogo Akademika N.F. Gamalei" Ministerstva Zdravookhranenija Rossijskoj Federatsii Im. N.F. Gamalei" Minzdrava Rossii)

METHOD FOR OBTAINING IMMUNOGENIC COMPOSITION BASED ON Ag85A-DBD HYBRID PROTEIN AND DEXTRANE; pAg85A-DBD RECOMBINANT PLASMIDE; Escherichia coli [pREP4, pAg85A-DBD] STRAIN; Ag85A-DBD CHIMERIC PROTEIN Russian patent published in 2014 - IPC C12N15/00 C12N1/21

Abstract RU 2520078 C1

FIELD: biotechnologies.

SUBSTANCE: invention represents Escherichia coli M15 [pREP4, pAg85A-DBD] strain - producer of Ag85A-DBD chimeric protein, as well as a method for immobilisation, concentration and cleaning of protein obtained based on dextrane. Invention relates to a method for obtaining immunogenic composition based on Ag85A-DBD recombinant protein mixed with dextrane and to Ag85A-DBD recombinant protein itself.

EFFECT: invention allows obtaining strain - producer providing high level of production of stable immunogenic proteins that can be obtained, immobilised and cleaned at a single stage, and obtaining effective immunogenic compositions against tuberculosis.

4 cl, 2 dwg, 1 tbl, 5 ex

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RU 2 520 078 C1

Authors

Tkachuk Artem Petrovich

Ljashchuk Aleksandr Mikhajlovich

Aksenova Ekaterina Ivanovna

Poletaeva Nina Nikolaevna

Galushkina Zoja Mikhajlovna

Sergienko Ol'Ga Vasil'Evna

Apt Aleksandr Solomonovich

Karjagina-Zhulina Anna Stanislavovna

Lunin Vladimir Glebovich

Gintsburg Aleksandr Leonidovich

Dates

2014-06-20—Published

2013-04-25—Filed