FIELD: biotechnology.
SUBSTANCE: proposed vaccine comprises recombinant pseudo-adenovirus particles on the basis of human adenovirus of fifth serotype bearing under the control of an inducible promoter, the full-length M2 protein gene and the full length protein gene NP, modified PEST motif and removing a nuclear localization signal, interconnected nucleotide sequence of FMDV 2A peptide. Vaccine is prepared by creating a consensus amino acid sequences of M2, and NP protein, NP protein sequence modifications PEST motif and removing a nuclear localization signal to obtain a genetic construct M2-2A-NP-PEST, the genes expressing these proteins under the control of a promoter inducible Tet-off. Preparation of plasmid pShuttle-tet-M2NP-PEST cloning M2-2A-NP-PEST genetic construct a shuttle plasmid carrying the regulatory elements Tet-off promoter to give plasmid pAd5-tet-M2NP-PEST homologous recombination using the plasmid pShuttle-tet-M2NP-PEST with a plasmid containing the genome of a recombinant human adenovirus of fifth serotype transfection plasmid pAd5-tet-M2NP-PEST in 293NEK cell multiplication, accumulation of particles of pseudo-adenovirus recombinant Ad5-tet-M2NP-PEST in the presence of doxycycline, the concentration and purification of these recombinant of pseudo-adenovirus particles using ultrafiltration and high performance liquid chromatography. Bringing pharmaceutically acceptable buffer concentration to 106-109 PFU/ml. The resulting preparation immunogenic and protective activity against influenza A virus of the following subtypes: H1N1, H2N3, H3N2, H5N2, H9N2.
EFFECT: invention can be used as a candidate universal vaccine against influenza A viruses of human and birds.
6 cl, 27 dwg, 2 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BROAD-SPECTRUM M2 PROTEIN ECTODOMAIN AVIAN INFLUENZA TYPE A VACCINE | 2014 |
|
RU2571944C1 |
| STRAIN OF A RECOMBINANT PSEUDO ADENOVIRAL PARTICLE BASED ON HUMAN ADENOVIRUS GENOME OF SEROTYPE 5 Ad5-tetOFF-E3-HA125 SEROTYPE, CARRYING HAEMAGGLUTININ CONSENSUS GENE OF INFLUENZA VIRUS SUBTYPES H1, H2, H5 FOR PRODUCING INFLUENZA IMMUNOGENIC PREPARATIONS, METHOD FOR PRODUCING GENE | 2018 |
|
RU2713722C1 |
| IMMUNOBIOLOGICAL AGENT AND A METHOD FOR USE THEREOF FOR INDUCING SPECIFIC IMMUNITY TO THE MIDDLE EASTERN RESPIRATORY SYNDROME VIRUS (VERSIONS) | 2018 |
|
RU2709659C1 |
| RECOMBINANT PSEUDO-ADENOVIRAL PARTICLE STRAIN EXPRESSING THE CHLAMYDIA TRACHOMATIS CHIMERIC GENE MBL-CT666, A METHOD FOR PREPARING IT, AN IMMUNOGENIC COMPOSITION FOR PROTECTION AGAINST HUMAN UROGENITAL CHLAMYDIOSIS | 2019 |
|
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| IMMUNOGENIC COMPOSITION BASED ON RECOMBINANT PSEUDO ADENOVIRAL PARTICLES, AS WELL AS BASED ON PROTEIN ANTIGENS AND A METHOD FOR PRODUCING AN IMMUNOGENIC COMPOSITION | 2018 |
|
RU2691302C1 |
| RECOMBINANT PSEUDO-ADENOVIRUS PARTICLE BASED ON GENOME OF HUMAN ADENOVIRUS OF SEROTYPE 5, PRODUCING INFLUENZA HEMAGGLUTININ OF STRAIN A/Brisbane/59/2007(H1N1) AND METHOD OF ITS USE | 2012 |
|
RU2523599C1 |
| RECOMBINANT PSEUDO ADENOVIRAL PARTICLE BASED ON HUMAN BEING ADENOVIRUS GENOME OF 5-TH SEROTYPE FOR INDUCTION OF SPECIFIC IMMUNITY TO INFLUENZA VIRUS A OF H1N1 SUBTYPE AND METHOD OF ITS USE AS COMPONENT FOR VACCINE PRODUCTION | 2012 |
|
RU2507257C1 |
| RECOMBINANT PSEUDO ADENOVIRAL PARTICLE BASED ON HUMAN BEING ADENOVIRUS GENOME OF 5-TH SEROTYPE, PRODUCING HAEMAGGLUTININ OF INFLUENZA VIRUS OF B/Brisbane/60/2008 STRAIN AND METHOD OF ITS USE FOR INDUCTION OF SPECIFIC IMMUNITY TO INFLUENZA VIRUS B | 2012 |
|
RU2507259C1 |
| RECOMBINANT PSEUDO ADENOVIRAL PARTICLE BASED ON HUMAN BEING ADENOVIRUS GENOME OF 5-TH SEROTYPE FOR INDUCTION OF SPECIFIC IMMUNITY TO INFLUENZA VIRUS A OF H3N2 SUBTYPE AND METHOD OF ITS USE | 2012 |
|
RU2507258C1 |
| EXPRESSION VECTOR BASED ON HUMAN ADENOVIRUS 5 SEROTYPE, INDUCING CROSS-PROTECTIVE IMMUNITY TO INFLUENZA A VIRUSES OF SUBTYPE H1, AND A PHARMACEUTICAL COMPOSITION BASED ON IT | 2023 |
|
RU2802753C1 |
