FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identification of species of mutton and beef in food raw materials, fodder and food products, including plastic flasks and test tubes, a thermostable enzyme Tag-polymerase, a reaction buffer, a mixture of four deoxynucleotide triphosphate, mutton (Ovis) DNA segment and beef (Bos) regions specific for oligonucleotide primers, probes, internal control sample in the form of bacteriophage suspension and positive control samples containing DNA fragments mutton (Ovis) and beef (Bos) genes, according to the invention for the internal control sample a bacteriophage T4 suspension with concentration of 5×10 copies of nucleotide sequences per 1 mcl, and for a positive control sample – a mixture containing fragments of mutton genomes (Ovis), beef (Bos) and bacteriophage T4, taken in ratio 1:1:1.
EFFECT: invention makes it possible to increase accuracy of beef or mutton species identity identification.
1 cl, 5 dwg, 5 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR IDENTIFICATION OF SPECIES IDENTITY OF MUTTON AND BEEF IN FOOD RAW MATERIALS, FODDER AND FOOD PRODUCTS | 2018 |
|
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RU2823069C1 |
