TEST SYSTEM FOR DETECTING DNA OF DONKEY (EQUUS ASINUS) TISSUE IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention represents a test system for identification of DNA of donkey (Equus asinus) tissue in dry fodder and meat semi-products, including a buffer for polymerase chain reaction, mixture for its carrying out, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes, specific for section of animal's genome and for internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×10³ phage particles per 1 mcl, a negative control sample which is a mixture of recombinant plasmid DNA, containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome with a nucleotide sequence: T4F TACATATAAATCACGCAAAGC – forward primer; T4R TAGTATGGCTAATCTTATTGG – reverse primer; T4P FAM ACATTGGCACTGACCGAGTTC BHQ1 – probe, taken in volume ratio of 1:1, according to invention for internal control sample phage lysate of bacteriophage T4 is used, and for positive control sample fragments of genomes of native bacteriophage T4 and donkey (Equus asinus) with the following nucleotide sequence are used: Donk F: 5'-CATATCTTAATCCCAATAATAGAC-3' – forward primer; Donk R: 5'-AGTATTCGTTCTGATATAGGC-3' – reverse primer; Donk P: FAM-5'-ATCTTATACTGGACTATTCTATCGAC-3 – BHQ1 – probe.

EFFECT: invention is used to expand functional capabilities and increase accuracy of identification of species identity, simplify sample preparation process.

1 cl, 5 tbl