DETERMINING THE ACTIVITY OF THE CLASSICAL PATHWAY OF THE COMPLEMENT SYSTEM IN A FIBRINOGEN COAGULATION TEST Russian patent published in 2019 - IPC G01N33/539 C12Q1/56 

FIELD: medicine.

SUBSTANCE: invention relates to medicine. Disclosed is a method for determining complement activity on a classical pathway in a fibrinogen coagulation test, involving use of human blood serum as a complement source and treated with 9.1 % polyvinylpyrrolidone with molecular weight of 35,000 citrated plasma as a prothrombin complex source with factor XII and fibrinogen. Degree of fibrinogen coagulation is determined as a difference in turbidity variation of sample at 450 nm after 20-minute incubation at 37 °C. Coagulation at the level of up to 20 % is considered to be low complement activity, from 20 % to 49 % – average complement activity, and more than 49 % – as high complement activity in the fibrinogen coagulation test.

EFFECT: invention provides extending the range of laboratory screening tests for diagnosing hypercoagulation caused by high functional activity of the classical pathway of the complement system in a fibrinogen coagulation test.

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