POLYSACCHARIDE AND ITS DERIVATIVES WITH ACTIVITY TO FICOLIN-3, METHOD FOR OBTAINING AND APPLICATION THEREOF Russian patent published in 2016 - IPC C08B37/00 

FIELD: chemistry.

SUBSTANCE: polysaccharide possesses affinity to ficolin-3 and is characterised by presence of oligosaccharide repeating unit of general formula (I). In formula (I) n is integer number from 0 to 100, preferably smaller than 50; PD_t stands for oligosaccharide repeating unitPD1_t of formula

(II),

oligosaccharide repeating unit PD2_t of formula

(III)

or oligosaccharide repeating unit PD3_t of formula

, (IV),

PD stands for oligosaccharide repeating unit PD1 of formula

,(V),

oligosaccharide repeating unit PD2 of formula

,(VI),

or oligosaccharide repeating unit PD3 of formula

, (VII)

[Hep]-Kdo stands for oligosaccharide repeating unit of formula

(VIII).

Method for obtaining claimed polysaccharide includes cultivation of H. alvei strain and separation of bacterial lipopolysaccharide. Obtained lipopolysaccharide is cleaved and polysaccharide fraction is separated. Polysaccharide fraction, containing polysaccharides, formed of repeating units with molecular weight in the range from 2400 to 26000 kDa, is separated. Obtained polysaccharides are optionally reduced, in particular in presence of NaBH₄, and preferably conjugated with known carrier-protein or chromatographic medium.

EFFECT: invention makes it possible to obtain ligands, which specifically find with ficolin-3.

14 cl, 10 dwg, 3 tbl, 13 ex