FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is a recombinant nucleic acid molecule for producing alanine in a recombinant E. coli microorganism, having a sequence, selected from a nucleic acid molecule group comprising a sequence of SEQ ID NO: 45 or molecules of nucleic acid coding polypeptide, which is at least 80 % identical to polypeptide with SEQ ID NO: 46, wherein the gene codon, denoted in the first group corresponding to position 286–288 in SEQ ID NO: 45, codes amino acid glutamic acid, or amino acid of proteins encoded by genes, designated in the second group, corresponding to position 96 in SEQ ID NO: 46 is glutamic acid.
EFFECT: invention can be used in pharmaceutical industry.
33 cl, 25 dwg, 1 tbl, 9 ex
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Authors
Dates
2019-12-05—Published
2014-12-08—Filed