IMPROVED EXPRESSION AND PROCESSING OF TRANSGENE Russian patent published in 2020 - IPC C12N15/90 C12N7/00 C07K16/00 C07K16/32 C12N15/85 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to a recombinant nucleic acid molecule for increasing the efficiency of producing a transgene expressed in a mammalian cell. Recombinant according to the present invention is designed so that it includes 5'-3'-transposon-specific end repeat of ITR, a nucleic acid coding a TEP transporter protein or a TEP RNA, and is also supplemented with an epigenetic regulatory element. In addition, a recombinant expression vector, a host cell, a method for expressing the encoded TEP, a cell transfection method and a cell transfection kit are described.

EFFECT: in comparison with known analogues, the invention demonstrates advantages in relation to expression value and quality, including high stability of transgene expression and, if necessary, transportation of the expressed product to the outside of the cell.

64 cl, 4 tbl, 25 dwg