FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, in particular to promoters of the expression of a Brachyury protein in poxviruses. An expression cassette for the expression of a Brachyury encoding sequence in a vector, a poxvirus vector containing it, and a method for expressing a sequence encoding a Brachyury antigen in a vector are proposed. The expression cassette contains (a) a nucleic acid sequence containing a promoter active as a poxvirus promoter and containing a sequence SEQ ID NO: 9 or a subsequence SEQ ID NO: 9, which contains any of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and (b) a heterologous sequence encoding the Brachyury antigen functionally associated with the promoter, wherein the expression of the encoding sequence is controlled by the promoter.
EFFECT: inventions make it possible to enhance the expression of the Brachyury antigen used in immunotherapy.
18 cl, 6 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMBINATION THERAPY FOR CANCER TREATMENT USING RECOMBINANT MVA AND ANTIBODIES INTRODUCED INTRAVENOUSLY | 2018 |
|
RU2795103C2 |
| NGF ANTAGONISTS FOR MEDICAL USE | 2020 |
|
RU2829812C2 |
| T-CELL RECEPTORS RECOGNIZING R175H OR Y220C MUTATION IN p53 | 2020 |
|
RU2830061C2 |
| IL-12 HETERODIMERIC FC FUSION PROTEINS | 2019 |
|
RU2819097C2 |
| RECOMBINANT VECTORS EXPRESSING AVIAN INFLUENZA VIRUS ANTIGENS AND APPLICATIONS THEREOF | 2017 |
|
RU2761869C2 |
| FUSION PROTEIN COMPRISING IL-12 AND ANTI-FAP ANTIBODY, AND USE THEREOF | 2021 |
|
RU2831612C1 |
| INSECTICIDE PROTEINS OF PLANT ORIGIN AND THEIR APPLICATION METHODS | 2016 |
|
RU2762832C2 |
| VERSIONS OF Fc-REGION WITH ALTERED BINDING TO NEONATAL Fc-RECEPTOR (FcRn) FOR USE IN VETERINARY SCIENCE | 2019 |
|
RU2830231C2 |
| VERSION FC AND ITS PRODUCTION | 2021 |
|
RU2828852C1 |
| FUSED PROTEIN CONSTRUCTS FOR COMPLEMENT-RELATED DISEASE | 2019 |
|
RU2824402C2 |
