FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, in particular to promoters of the expression of a Brachyury protein in poxviruses. An expression cassette for the expression of a Brachyury encoding sequence in a vector, a poxvirus vector containing it, and a method for expressing a sequence encoding a Brachyury antigen in a vector are proposed. The expression cassette contains (a) a nucleic acid sequence containing a promoter active as a poxvirus promoter and containing a sequence SEQ ID NO: 9 or a subsequence SEQ ID NO: 9, which contains any of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and (b) a heterologous sequence encoding the Brachyury antigen functionally associated with the promoter, wherein the expression of the encoding sequence is controlled by the promoter.
EFFECT: inventions make it possible to enhance the expression of the Brachyury antigen used in immunotherapy.
18 cl, 6 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMBINATION THERAPY FOR CANCER TREATMENT USING RECOMBINANT MVA AND ANTIBODIES INTRODUCED INTRAVENOUSLY | 2018 |
|
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| IL-12 HETERODIMERIC FC FUSION PROTEINS | 2019 |
|
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| RECOMBINANT VECTORS EXPRESSING AVIAN INFLUENZA VIRUS ANTIGENS AND APPLICATIONS THEREOF | 2017 |
|
RU2761869C2 |
| INSECTICIDE PROTEINS OF PLANT ORIGIN AND THEIR APPLICATION METHODS | 2016 |
|
RU2762832C2 |
| FUSED PROTEIN CONSTRUCTS FOR COMPLEMENT-RELATED DISEASE | 2019 |
|
RU2824402C2 |
| IL4/IL13 RECEPTOR MOLECULE FOR VETERINARY USE | 2018 |
|
RU2795591C2 |
| ISOLATION OF A NEW PESTIVIRUS CAUSING CONGENITAL TREMOR A | 2017 |
|
RU2765658C2 |
| EMBODIMENTS OF IGG-Fc FOR USE IN VETERINARY SCIENCE | 2018 |
|
RU2814952C2 |
| FUSED MOLECULES DERIVED FROM CHOLIX-TOXIN FOR ORAL DELIVERY OF BIOLOGICALLY ACTIVE LOADS | 2015 |
|
RU2723178C2 |
| POLYVALENT AND POLYSPECIFIC OX40-BINDING FUSED PROTEINS | 2017 |
|
RU2773052C2 |
