FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, in particular to promoters of the expression of a Brachyury protein in poxviruses. An expression cassette for the expression of a Brachyury encoding sequence in a vector, a poxvirus vector containing it, and a method for expressing a sequence encoding a Brachyury antigen in a vector are proposed. The expression cassette contains (a) a nucleic acid sequence containing a promoter active as a poxvirus promoter and containing a sequence SEQ ID NO: 9 or a subsequence SEQ ID NO: 9, which contains any of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 8, and (b) a heterologous sequence encoding the Brachyury antigen functionally associated with the promoter, wherein the expression of the encoding sequence is controlled by the promoter.
EFFECT: inventions make it possible to enhance the expression of the Brachyury antigen used in immunotherapy.
18 cl, 6 dwg, 1 tbl, 4 ex
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ISOLATION OF A NEW PESTIVIRUS CAUSING CONGENITAL TREMOR A | 2017 |
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EMBODIMENTS OF IGG-Fc FOR USE IN VETERINARY SCIENCE | 2018 |
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FUSED MOLECULES DERIVED FROM CHOLIX-TOXIN FOR ORAL DELIVERY OF BIOLOGICALLY ACTIVE LOADS | 2015 |
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POLYVALENT AND POLYSPECIFIC OX40-BINDING FUSED PROTEINS | 2017 |
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RU2773052C2 |
SOLUBILIZED APIRASES, METHODS AND APPLICATIONS | 2019 |
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RU2791992C2 |
Authors
Dates
2021-08-24—Published
2016-07-28—Filed