FIELD: biotechnology.
SUBSTANCE: invention represents a method for sterilization of green plant explants prior to introduction into culture in vitro, including preparation and sterilization. When harvesting plant material, shoot tips of 10–15 cm of actively vegetating plants are used, and sterilization of explants is performed successively in two steps: at first 70–75 % with aqueous ethanol solution for 30 seconds, and then – 20–30 % aqueous solution of preparation "Dezavid+" with exposure for 10 minutes and washing 3-4 times with sterile distilled water for 5 minutes. Invention provides higher survival rate and regeneration of meristems from tops of young green sprouts by reducing their contamination and necrosis.
EFFECT: improves safety and efficiency of micropropagation of plants in vitro.
1 cl, 3 tbl
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