TEST SYSTEM FOR IDENTIFICATION OF DNA OF DOMESTIC CAT TISSUE (FELIS SILVESTRIS CATUS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identification of domestic cat tissue DNA (Félis silvéstris cátus) in dry fodders and semi-finished meat products, including a polymerase chain reaction buffer, mixture for its carrying out, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes, specific for section of animal's genome and for internal control sample; mixture of enzymes from DNA polymerase with enzyme inhibiting enzymes, TAQ POLYMERASE, internal control sample in the form of suspension of bacteriophage T4 with concentration of 5 × 10³ phage particles per 1 mcl, negative control sample, which is a mixture of recombinant plasmid DNA, containing a fragment of an animal's genome and a fragment of the bacteriophage T4 genome with a nucleotide sequence: T4F: 5'-TACATATAAATCACGCAAAGC-3' - forward primer; T4R: 5'-TAGTATGGCTAATCTTATTGG-3' - reverse primer; T4P: CY-5'-ACATTGGCACTGACCGAGTTC-3'-BHQ1 - probe, taken in volume ratio of 1:1, according to invention for internal control sample is phagolysate of bacteriophage T4, and for the positive control sample fragments of genomes of native bacteriophage T4 and domestic cat (Félis silvéstris cátus) are used with the following nucleotide sequence: Félis F ATTCggCCTACATCCgTgAC - forward primer; Félis R AgAAgACCCCTgCTACgACT - reverse primer; Félis P R6G-CTTgAgTggAgTAgggCgg-BHQ1 - probe.

EFFECT: invention allows to expand functional capabilities and increase accuracy of species identity identification, simplify sample preparation process.

1 cl, 5 tbl