FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used for recovery of intestinal microflora in diseases of gastrointestinal tract associated with disruption of digestion, microflora and presence of intestinal infections. Method involves extraction of percoll by filtration through strains with 100 mcm cells and gradient centrifugation in phosphate-salt solution and in gradient fluid. Cells are then washed with 1 % phosphate-salt solution and concentrated by centrifugation at 13,000 rpm for 15 minutes and frozen in cryo-medium with embryonal bovine serum with storage at -80 °C.
EFFECT: invention enables to preserve microbiota of intestinal microflora in diseases of gastrointestinal tract.
1 cl, 3 dwg
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