METHOD FOR DETERMINING DNA OF NODULAR DERMATITIS VIRUS (LSDV) IN BIOLOGICAL MATERIAL OF ANIMALS BY PCR WITH ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS IN AGAROSE GEL Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a method for determining DNA of nodular dermatitis virus (LSDV) in biological material of animals by PCR with electrophoretic detection of amplification products in agarose gel, involving DNA isolation of infectious disease agent from biological material of infected animal by sorption method, polymerase chain reaction with fluorescent detection with 40 cycles of amplification using oligonucleotide primer DNA-specific DNA infectious agent causative agent DNA, fluorescent dye, an internal control sample in form of suspension of bacteriophage T4 with concentration of 5 × 10³ phage particles per 1 mcl and a positive control sample in the form of a mixture containing in the same volume ratio fragments of genomes of the causative agent of the infectious animal disease and bacteriophage T4, according to the invention, DNA of the nodular dermatitis virus (LSDV) is recovered in the biological material of animals, performing electrophoretic detection of the amplification products in the agarose gel and obtaining an electrophoregram of DNA with specific bands of the amplified product, visualizing their colouring with a fluorescent dye, according to which recording and interpretation of the PCR results is carried out by the presence or absence of a specific strand of a DNA fragment of the nodular dermatitis virus, coinciding in size with the band of the fragment of the same DNA, amplified from a positive control sample, wherein a mixture of recombinant plasmid DNA containing a DNA fragment of the nodular dermatitis virus and a fragment of the bacteriophage T4 genome with the following nucleotide sequences is used.

EFFECT: invention widens functional capabilities in detecting a residual amount of DNA of nodular dermatitis virus.

1 cl, 6 tbl