TEST SYSTEM FOR IDENTIFICATION OF DNA TISSUE OF COMMON QUAIL (COTURNIX COTURNIX) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention represents a test system for DNA identification of common quail (Coturnix coturnix) tissue in dry fodders and semi-finished meat products, including a polymerase chain reaction buffer, mixture for its carrying out, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes, specific for section of animal's genome and for internal control sample; a mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, an internal control sample in the form of suspension of bacteriophage T4 with concentration of 5×10³ phage particles per 1 mcl, negative control sample, which is a mixture of recombinant plasmid DNA, containing a fragment of an animal's genome and a fragment of a bacteriophage T4 genome with a nucleotide sequence: T4F TACATATAAATCACGCAAAGC – forward primer, T4R TAGTATGGCTAATCTTATTGG – reverse primer, T4P FAM ACATTGGCACTGACCGAGTTC BHQ – probe, taken in volumetric ratio of 1:1, the internal control sample is presented with phagolysate of bacteriophage T4, and for the positive control sample – fragments of genomes of native bacteriophage T4 and quail tissue Coturnix coturnix) with the following nucleotide sequence: C.cot-F: 5'-CTAGGAGGCGTACTTGC-3' – forward primer; C.cot-R: 5'-GTGGGCGGAATGTTATG-3' – reverse primer; C.cot-Z: 5'-CAGTACTTATCCTCCTTCTAATCC-3' – probe.

EFFECT: invention broadens functional capabilities and accuracy of identification of species identity, simplifies sample preparation process.

1 cl, 5 tbl