FIELD: biotechnology.
SUBSTANCE: method for assessing the reproductive activity of influenza viruses in a trivalent and quadrivalent live influenza vaccine, based on a one-step real-time RT-PCR using hydrolysable oligonucleotide fluorescent probes. Real-time RT-PCR using the developed specific primers and oligonucleotide probes is sensitive and specific for studying reproduction of vaccine viruses in MDCK cell culture in a trivalent or quadrivalent vaccine. Use of real-time RT-PCR makes it possible to study mutual influence of components of polyvalent LIV even at reduced level of reproduction of vaccine viruses in cells of mammals in comparison with reproduction in CE. Developed method enables assessing reproduction of influenza A and B vaccine viruses in a trivalent vaccine in samples obtained from respiratory tracts of laboratory animals and patients after immunization with trivalent LIV.
EFFECT: method enables assessing the mutual influence of influenza B viruses in Victoria and Yamagata antigenic lines for inclusion in polyvalent vaccines.
1 cl, 3 tbl, 8 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| INFLUENZA VIRUS STRAIN FOR PRODUCING LIVE INFLUENZA INTRANASAL VACCINE FOR ADULTS AND CHILDREN | 2011 |
|
RU2464311C1 |
| REASSORTANT RN A9-SWINE A(H7N1) INFLUENZA VIRUS STRAIN FOR NEURAMINIDASE ANTIBODY ASSAY IN INFLUENZA INFECTION AND VACCINATION | 2010 |
|
RU2428476C1 |
| INFLUENZA VIRUS STRAIN A/Hongkong/1/68/162/35 (H3N2) - UNIVERSAL DONOR OF INTERNAL GENES FOR REASSORTANTS, AND REASSORTANT STRAINS A/SPB/GK/09 (H1N1) AND A/HK/Astana/6:2/2010 (H5N1) PREPARED THEREOF | 2011 |
|
RU2511431C2 |
| INFLUENZA VIRUS STRAIN FOR PRODUCING LIVE AND INACTIVATED INFLUENZA VACCINE | 2011 |
|
RU2464309C1 |
| STRAIN OF INFLUENZA VIRUS GKV 2389 FOR PRODUCTION OF LIVING INTRANASAL AND INACTIVATED INFLUENZA VACCINE | 2006 |
|
RU2318871C1 |
| RECOMBINANT VACCINE STRAIN FOR LIVE INTRANASAL VACCINE PROVIDING COMBINED PREVENTION OF INFLUENZA AND CORONAVIRUS INFECTIONS | 2022 |
|
RU2782531C1 |
| STRAIN OF VIRUS OF FLU A/17/WISCONSIN/05/84 (H3N2) FOR MANUFACTURE OF LIVE INFLUENZAL INTRANASAL VACCINE FOR ADULTS AND CHILDREN | 2007 |
|
RU2340671C1 |
| REASSORTANT A/17/NEW CALEDONIA/99/79(H1N1) INFLUENZA VIRUS STRAIN FOR PRODUCING LIVE INFLUENZA INTRANASAL VACCINE | 2011 |
|
RU2464310C1 |
| REASSORTANT INFLUENZA VIRUS STRAIN RN9/13-HUMAN A(H6N9) FOR NEURAMINIDASE ANTIBODY ASSAY IN INFLUENZA INFECTION AND VACCINATION | 2014 |
|
RU2587629C1 |
| STRAIN OF INFLUENZA VIRUS A/17/VICTORIA/2011/89 (H3N2) FOR PRODUCTION OF LIVE INFLUENZA INTRANASAL VACCINE FOR ADULTS AND CHILDREN | 2012 |
|
RU2532844C2 |
