FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, virology and medicine. Diagnostic influenza virus strain RN9/13-human A (H6N9) is obtained by crossing apatogenic bird flu virus a/silver gull/Sarma/51 c/06 (H6N1) with cold-adapted vaccine strain a/17/Anui/2013/61(H7N9) based on attenuation donor A/Leningrad/134/17/57 (H2N2). Strain has neuraminidase influenza virus subtype N9 A/Anui/1/2013(H7N9) and avian influenza virus hemagglutinin and/silver gull/Sarma/51c/06(H6N1). Strain RN9/13-human A(H6N9) actively propagates in developing chicken embryos at optimum temperature of 33 °C, which enables to accumulate viral material for further purification and concentration.
EFFECT: strain RN9/13-human A(H6N9) can be used for detection of antibodies to influenza virus neuraminidase N9 in blood serum using solid-phase reaction of inhibiting neuraminidase activity and post-adsorption micro-neutralisation reaction in MDCK cell culture.
1 cl, 2 dwg, 1 tbl, 1 ex
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