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2 737 487

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IPC

C12N15/85(2006-01-01)

C07K14/475(2006-01-01)

A61K48/00(2006-01-01)

A61P9/10(2006-01-01)

(21) (22)

Application

2019135603, 2019-11-06

(24)

Start date

2019-11-06

(22)

Actual filing date

2019-11-06

(45)

Published

2020-12-01

(72)

Inventor

Slobodkina Ekaterina AleksandrovnaKaragyaur Maksim NikolaevichBalabanyan Vadim YurevichParfenova Elena ViktorovnaMakarevich Pavel IgorevichAkopyan Zhanna AlekseevnaTkachuk Vsevolod Arsenevich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennostyu I Kletochnaya Terapiya" I Kletochnaya Terapiya")

GENETIC ENGINEERING STRUCTURE FOR ANGIOGENESIS STIMULATION Russian patent published in 2020 - IPC C12N15/85 C07K14/475 A61K48/00 A61P9/10

Abstract RU 2737487 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to gene engineering structures for angiogenesis stimulation, and can be used in medicine for treating diseases caused by blood tissue disorders. Disclosed is a plasmid bicistronic construct carrying the human hepatocyte growth factor hHGF gene and the vascular endothelial growth factor hVEGF165 gene with two separate promoters for each of the genes: cytomegalovirus promoter - pCMV for HGF gene and gene promoter chicken β-actin - pCAG for VEGF165 gene. Bicistronic structure can be used as a medicinal agent for stimulating angiogenesis, growth and remodeling of vessels, as well as restoration of blood supply in ischemic tissues.

EFFECT: invention enables achieving optimal concentrations and ratio of angiogenic growth factors (AGF).

6 cl, 7 tbl, 1 ex, 6 dwg

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RU 2 737 487 C1

Authors

Slobodkina Ekaterina Aleksandrovna

Karagyaur Maksim Nikolaevich

Balabanyan Vadim Yurevich

Parfenova Elena Viktorovna

Makarevich Pavel Igorevich

Akopyan Zhanna Alekseevna

Tkachuk Vsevolod Arsenevich

Dates

2020-12-01—Published

2019-11-06—Filed