FIELD: biotechnology.
SUBSTANCE: invention relates to the biotechnology. Disclosed is a method of producing and purifying a biologically active substance produced by bifidobacteria. Method involves cultivation of a certain type of bifidobacteria, separation of microbial mass with subsequent treatment of cold supernate dimethyl ketone, separating the precipitate, dissolving it in a buffer solution and Sephadex LH-20 chromatography with re-chromatography on an affine sorbent.
EFFECT: method provides efficiency of separation, selectivity of purification of biologically active substances produced by bifidobacteria.
1 cl, 1 ex
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