FIELD: biotechnology.
SUBSTANCE: method for purification of P436(KS) mundticin is proposed, which includes cultivating the producer strain Enterococcus mundtii "GKPM - Obolensk" B-8925 for 12 hours in a bioreactor with a Brucella broth nutrient medium with the addition of meat extract and lactose and adding the sorbent CM-sephadex C25 10% of the volume into a bioreactor with a nutrient medium immediately before cultivation, followed by autoclaving of the biomass together with the sorbent at 0.5 atm for 15 min. Then the sorbent is taken, washed with phosphate-buffered saline with the addition of ethanol, transferred to a chromatographic column, followed by elution of the sorbed mundticin with a buffer, and the resulting one-stage purification product is further purified using HPLC by reverse-phase chromatography on a C18 column.
EFFECT: invention provides an increase in the purity and yield of P436 mundticin suitable for industrial use.
1 cl, 7 dwg, 5 tbl, 4 ex
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