METHOD OF PRODUCING A FUNCTIONAL PRODUCT FROM MYTILUS GALLOPROVINCIALIS MUSSELS Russian patent published in 2021 - IPC A23L33/10 A23L17/50 C11B1/10 

FIELD: biologically active additives.

SUBSTANCE: invention relates to biologically active additives. A method for producing a functional product of Mytilus galloprovincialis mussels, involving temperature stimulation of mussels spawning; obtaining of sexual products; artificial insemination, excluding polyspermy; obtaining fertilized eggs; separation of blastomeres in sea water with salinity of 18 ‰ into individual cells with sodium citrate solution at its concentration in sea water of 0.1 M; selection of blastomeres. For product containing mussel stem cells only, for stimulation of spawning, filtered sea water with temperature of 23-25°C is used, artificial insemination is carried out at temperature of 20-22°C, selection of embryo stem cells is performed not later than 60 min after fertilization, then, after blastomer separation into separate stem cells with the help of citric citrate, they are filtered and washed with distilled water, after which the obtained stem cells are filled with 96% ethanol in ratio 1:2 starting material : ethanol. A method for producing a functional product of Mytilus galloprovincialis mussels, involving temperature stimulation of mussels spawning; obtaining of sexual products; artificial insemination, excluding polyspermy; obtaining fertilized eggs. To produce a product containing stem cells and non-infertile sexual products, for stimulation of mussel spawning, filtered sea water with temperature of 23-25°C is used, artificial insemination is carried out in sea water at temperature of 20-22°C, embryo stem cells are selected at the stage of blastomer formation not later than 60 min after fertilization by sedimentation together with non-infertile sexual products at 1500 rpm, supernatant is drained; then, the residue containing stem cells and sexual products is washed repeatedly with distilled water at 1500 rpm, the aqueous layer is removed, after which 96% ethanol is poured in ratio 1:2 starting material : ethanol.

EFFECT: invention enables to obtain a product having biological activity with high content of polyunsaturated fatty acids, testosterone and amino acids.

2 cl, 2 ex