METHOD FOR CRYOCONSERVATION OF CELLS IN SEA INVERTEBRATES Russian patent published in 2008 - IPC A01N1/02 

FIELD: medicine, biotechnology.

SUBSTANCE: the present innovation deals with treating embryonic cells of sea invertebrates with cryoprotector mixture that includes lipids, sugars, antioxidants and saline solution. As lipids one should apply lipid extracts of sea hydrobionts at low temperatures of phase transitions which should be pre-treated with ultrasound. Cryoprotector mixture should used at a certain ratio of the components taken, weight%. Moreover, cryoprotector mixture should be additionally supplemented with 5-10%-dimethyl sulfoxide (DMSO). The innovation enables to increase the degree of conservation of cells in sea invertebrates without affecting their function activity after freezing-defrosting due to keeping the integrity and the stability of their cell membranes and provides the chance for evaluating cell conservation quantitatively.

EFFECT: higher efficiency.

1 cl, 2 dwg, 7 ex, 2 tbl