YEAST PRODUCING EKTOIN Russian patent published in 2021 - IPC C12N15/81 C12P17/12 

FIELD: ectoine bioproduction.

SUBSTANCE: group of inventions relates to the field of ectoine bioproduction. Proposed are recombinant yeast for the production of ectoine, in the genome of which at least one nucleic acid encoding aspartokinase is overexpressed and/or is under the control of an inducible or repressed promoter and/or at least one nucleic acid encoding aspartate kinase is overexpressed and/or is under control of an inducible or repressed promoter. Also, in the genome of said yeast, at least one nucleic acid encoding dehydrogenase of aspartic acid semialdehyde and/or at least one nucleic acid encoding aspartic acid semialdehyde dehydrogenase, which can be used as a coenzyme as nicotinamide adenine dinucleotide (NAD) and nicotinamide (NADP), is overexpressed and/or under the control of an inducible or repressed promoter. Also in the genome of said yeast at least one nucleic acid encoding diaminobutyrate aminotransferase is overexpressed and/or under the control of an inducible or repressed promoter. Also in the genome of said yeast at least one nucleic acid encoding homoserine-O-acetyltransferase METX is overexpressed and/or under the control of an inducible or repressed promoter, and/or at least one nucleic acid encoding diaminobutyric acid acetyltransferase is overexpressed and/or under the control of an inducible or repressible promoter. Also in the genome of said yeast at least one nucleic acid encoding ectoine synthase is overexpressed and/or under the control of an inducible or repressed promoter. Also in the genome of said yeast at least one, preferably all endogenous nucleic acids encoding homoserine dehydrogenase have been removed and/or disrupted, and/or at least one, preferably all nucleic acids encoding homoserine dehydrogenase are independently under the control of an inducible or repressible promoter, under the control of a weak promoter and/or in a destabilized form. Also, in the genome of said yeast, at least one nucleic acid encoding glutamate dehydrogenase using NADH and converting oxoglutarate into glutamate is overexpressed and/or under the control of an inducible or repressed promoter. Also proposed is a method for producing ectoine using said yeast and the use of said yeast for producing ectoine.

EFFECT: group of inventions provides highly efficient synthesis and secretion of ectoine.

15 cl, 2 ex