RECOMBINANT YEAST CELLS PRODUCING POLYLACTIC ACID AND THEIR USE Russian patent published in 2021 - IPC C12N15/54 C12N15/81 C12N9/10 C12N1/19 C12P7/56 C08G63/08 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to a recombinant yeast cell suitable for the production of polylactic acid (hereinafter – PLA), and its use. A recombinant yeast cell suitable for the production of PLA is proposed, containing and expressing a gene encoding lactyl-CoA-synthase and a gene encoding polyhydroxyalkanoate synthase (hereinafter – PHA) (PhaCp), additionally containing a peroxisomal targeted sequence (PTS). Lactyl-CoA-synthase is selected from a group consisting of CoA transferase, propionyl-CoA transferase (Pctp) or CoA ligase. At least one activity of lactic acid oxidoreductase is inactivated in the cell, preferably a gene encoding lactic acid dehydrogenase is inactivated, more preferably a gene encoding D-lactic acid dehydrogenase is inactivated. A recombinant yeast cell is designed for the expression of lactyl-CoA-synthase in cytosol and for the expression of PHA synthase in peroxisome of the specified cell. A method for producing PLA using the specified cell, enzymatic broth for producing PLA containing the specified cell are also proposed.

EFFECT: group of inventions provides the production of high amounts of PLA.

13 cl, 10 dwg, 5 tbl, 4 ex