METHOD FOR SIMULATING ATHEROSCLEROSIS AGAINST BACKGROUND OF DIABETES MELLITUS IN EXPERIMENT Russian patent published in 2021 - IPC G09B23/28 

FIELD: experimental medicine.

SUBSTANCE: invention relates to experimental medicine, namely to pathophysiology and cardiovascular surgery, and can be used to study the mechanisms of occurrence and development of atherosclerotic damage to the cardiovascular system against the background of diabetes mellitus in experimental animals. The method for simulating atherosclerosis against the background of diabetes mellitus in the experiment is that outbred male rabbits are daily taken throughout the experiment, products containing 0.8-1 g of cholesterol per 1 kg of body weight with free access to 5% glucose solution, average calorie content 4000-5000 kcal/day. After 3 weeks from the beginning of the diet, an analysis of the lipid and glycemic profile of the whole blood of rabbits is carried out with the determination of the atherogenic index to ascertain the fact of dyslipidemia, as well as a study of glycosylated hemoglobin. Then dithizone is injected intravenously at a dose of 25 mg/kg. 24 hours after the injection of dithizone, the level of glucose in the capillary blood of rabbits is determined using a glucometer and the glycosylated hemoglobin is re-examined. For 6 months from the start of the experiment, the rabbits continue to receive the indicated diet. The blood glucose level is adjusted by subcutaneous injection of short-acting insulin, maintaining it in the range of 10-12 mmol/l. After 6 months from the beginning of the experiment, the development of atherosclerosis against the background of diabetes mellitus in rabbits was confirmed by the study of blood biochemical parameters, ultrasound Doppler sonography of the arteries of the extremities, morphological examination of the heart and arteries after the animal was removed from the experiment.

EFFECT: invention makes it possible to simulate atherosclerosis against the background of diabetes mellitus.

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