METHOD FOR IMMOBILIZING MICROORGANISMS ON MONTMORILLONITE CLAYS Russian patent published in 2021 - IPC C12N11/00 C12N1/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and is a method for immobilizing microorganisms on montmorillonite clays. The method includes mixing pre-enriched and activated 10% hydrochloric acid montmorillonite clay with biological material in the form of biomass of microorganisms Lysobacter sp. Wherein, after activation, montmorillonite clay is washed with purified distilled water to a neutral medium, dried at a temperature of no more than 105°C and ground in a ball mill to a particle size of no more than 10 mcm; and biological material in the form of biomass of microorganisms Lysobacter sp. pre-fill with 0.05 M tris-hydrochloride buffer solution with pH 7.5 and mix, wherein, with stirring, the mixture is gradually heated to 45°C for 5 minutes, then cooled to room temperature and centrifuged, after which the supernatant is decanted and to the remainder of the treated biomass in the form of L-form Lysobacter sp. with constant mechanical stirring, the crushed clay is gradually added in the ratio of the carrier : biomass equal to 1:(2-4) at a temperature of 25°C; the mixture is thoroughly mixed for at least 40 minutes and then freeze-dried at a temperature of minus 40-45°C for 24 hours to a level of 3-7% moisture content of the composition.

EFFECT: invention allows extending the shelf life of bacterial cells of Lysobacter sp. on a carrier made of montmorillonite clay, with the possibility of ensuring the restoration of their activity when applied to the wound surface.

2 cl, 1 tbl, 5 ex