METHOD OF OBTAINING DRY FORM OF BIOPREPARATION FOR CLEANING TERRITORIES FROM OIL CONTAMINANTS AND OIL PRODUCTS Russian patent published in 2011 - IPC C12N1/26 C02F3/34 B09C1/10 

Abstract RU 2434059 C1

FIELD: chemistry.

SUBSTANCE: disclosed is a method of obtaining a dry form of a biopreparation based on Pseudomonas or Rhodococcus oil-decomposing microorganism for cleaning territories from oil and oil products. Pseudomonas or Rhodococcus bacteria are cultured in a liquid culture medium. The bacteria suspension is them mixed with a protective medium. The protective medium is prepared based on 0.05 M sodium-potassium phosphate buffer with pH 6.8, with the following ratio of components: 4% polyglucin, 10% saccharose, 4% thiourea, 2% ascorbic acid, followed by titration with 45% sodium hydroxide solution to pH 6.8-7.2. The obtained mixture is added to a sorbent - expanded pearlite sand. The preparation then undergoes contact drying at t=37°C until constant mass.

EFFECT: method increases survival rate of bacterial cells of oil-decomposing microorganisms Pseudomonas and Rhodococcus.

1 dwg, 2 tbl, 3 ex

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RU 2 434 059 C1

Authors

Petrikov Kirill Vladimirovich

Ovchinnikova Anastasija Alekseevna

Vetrova Anna Andrijanovna

Ponomareva Ol'Ga Nikolaevna

Filonov Andrej Evgen'Evich

Puntus Irina Filippovna

Samojlenko Vladimir Aleksandrovich

Jakshina Tat'Jana Vasil'Evna

Boronin Aleksandr Mikhajlovich

Dates

2011-11-20Published

2010-05-27Filed