METHOD FOR ISOLATION OF TUMOR CELLS FROM PERIPHERAL BLOOD Russian patent published in 2021 - IPC G01N33/48 G01N30/18 

FIELD: biology.

SUBSTANCE: invention relates to the field of biology and medicine, and is intended for the isolation of tumor cells from peripheral blood. Peripheral blood is diluted with buffer and filtered through a track membrane with pores under reduced pressure, on which antibodies to the surface antigens of secreted tumor cells, epithelial adhesion molecule (EpCAM), are preliminarily immobilized. The track membrane with the tumor cells trapped on it is poured with a solution that preserves the cell structure, which is human or animal blood serum, dried by rotating the track membrane around the axis, and cytological examination of tumor cells is carried out directly on the track membrane.

EFFECT: invention provides an increase in the reliability of determining the pathological nature of tumor cells circulating in the peripheral blood, due to the preservation and improvement of the quality of the cytological picture of the cells retained by the track membrane.

2 cl, 5 dwg, 4 ex