FIELD: biotechnology.
SUBSTANCE: group of inventions relates to isolation of rare cells from biological samples by filtration and to subsequent assay thereof. Disclosed is a method for non-invasive prenatal diagnostics of genetic diseases, including: (a) collection of a transcervical sample in the external pharynx of the endocervical canal — ectocervix, containing rare trophoblast cells in pregnant women; (b) immunolabelling and/or staining of cells with an alcyan blue solution; (c) isolation or concentration of rare trophoblast cells by passing the sample through a filter with a pore size, pore density, or thickness concentrating rare trophoblast cells; (d) genotyping of rare cells to identify trophoblast cells for further genetic assay thereof for the purpose of non-invasive prenatal diagnostics of genetic diseases. Also disclosed is a set for implementing said method.
EFFECT: group of inventions ensures isolation of cells by filtration, wherein rare cells are in a state suitable for multiple or even simultaneous analytical procedures.
13 cl, 4 dwg, 4 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR MULTI ANALYSES OF RARE CELLS EXTRACTED OR ISOLATED FROM BIOLOGICAL SAMPLES BY FILTERING | 2013 |
|
RU2641595C2 |
METHODS FOR CLASSIFYING PATIENTS WITH SOLID CANCER | 2017 |
|
RU2745730C2 |
MUTATED LENTIVIRAL ENV PROTEINS AND THEIR USE AS DRUGS | 2012 |
|
RU2654673C2 |
INDIVIDUALIZED ANTI-TUMOR VACCINES | 2012 |
|
RU2779946C2 |
RECOMBINANT VACCINE OF FELINE LEUKEMIA VIRUS CONTAINING OPTIMISED GENE OF FELINE LEUKEMIA VIRAL ENVELOPE | 2012 |
|
RU2591817C2 |
METHODS AND COMPOSITIONS FOR TREATING TUMORS | 2017 |
|
RU2741786C2 |
REPRESENTATIVE DIAGNOSIS | 2016 |
|
RU2743169C2 |
METHOD AND SYSTEM FOR MULTIPLEX PROFILING OF CHROMOSOMES IN BIOLOGICAL SAMPLES USING TARGET-SPECIFIC DNA PROBES | 2016 |
|
RU2741096C2 |
METHODS AND PHARMACEUTICAL COMPOSITIONS FOR TREATMENT OF KIDNEY CANCER | 2017 |
|
RU2752322C2 |
INDIVIDUALISED VACCINES FOR CANCER | 2012 |
|
RU2670745C9 |
Authors
Dates
2022-02-03—Published
2013-05-23—Filed