FIELD: medicine.
SUBSTANCE: mouse hybridoma called S79 is produced by fusion of spleen lymphocytes of a mouse immunised by the recombinant protein SURF-6 fused with glutathione-S-transferase, expressed and recovered from E coli cells with P3X63-Ag8.653 mouse myeloma cells in the ratio 3:1 by 50 % polyethylene glycol of molecular weight 1450. The produced hybridoma secretes a monoclonal antibody S79 specific to human nucleolus protein SURF-6 with a known amino acid sequence regardless of tissue or linear origin of cells. The antibody S79 does not react with SURF-6 in mouse cells NIH/3T3 and rat cells C6. The monoclonal antibody S79 produced by clone S79 can be used for detecting the SURF-6 in proliferative normal and tumour human lymphocytes, and also for detecting a possible contamination of human cell cultures by the cells of other species, including a mouse and a rat.
EFFECT: produced monoclonal antibody is used for specific detection of the human nucleolus protein by an immune-enzyme method, in the immunofluorescence and immunoblot reactions in human cells of various linear and fabric origin.
11 dwg, 6 ex
