FIELD: cell biology; medicine.
SUBSTANCE: present invention relates to the field of cell biology and medicine, in particular to a method for detecting residual, undifferentiated induced pluripotent stem cells (hereinafter – iPSC) in the culture of cells differentiated from iPSC. To implement the method according to the invention, the specified cells are first cultivated on substrate coated with laminin-521 and E-cadherin in a medium containing a Rho-associated protein kinase (ROCK) inhibitor containing super-spiral. Then, quantitative measurement of the expression of a marker of residual, undifferentiated iPSC is carried out in cultivated cells. Next, the expression of the marker in cultivated cells is compared with the expression of a marker in a control cell culture containing a known proportion of iPSC. It is concluded that there are no residual, undifferentiated iPSC in cultivated cells or that they are present in a smaller proportion than the known proportion of iPSC in the control cell culture in the case when a lower expression of the marker is detected in the cell culture than the expression of the marker in the control cell culture. The present invention also discloses a set for implementing the specified method, as well as a method for producing a therapeutic composition and a method for treating or preventing conditions in which an immunomodulatory effect is useful.
EFFECT: present invention allows one to obtain an improved method for detecting residual, undifferentiated PSC in the cell culture originating from iPSC, which is to be used in quality control in the production of therapeutic compositions containing cells originating from iPSC.
22 cl, 14 dwg, 8 tbl, 13 ex
