METHOD FOR EVALUATING THE EFFECTIVENESS OF LEPROSY THERAPY USING POLYMERASE CHAIN REACTION FOR AMPLIFICATION OF SPECIES-SPECIFIC SECTIONS OF THE LEPROSY, HUMAN OR MOUSE GENOMES Russian patent published in 2021 - IPC G01N33/50 C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and concerns a method for evaluating the effectiveness of leprosy chemotherapy by quantitative analysis of Mycobacterium leprae DNA in laboratory animal or human tissues, where in a real-time polymerase chain reaction using highly specific primers and hydrolyzable probes, a parallel amplification of a species-specific section of the carrier gene selected from a section of the human beta-actin gene (ACTB) or a section of the mouse B2-microglobulin gene (B2M) is performed, and a variable section of the RLEP genome of the pathogen, followed by the calculation of the ratio of the obtained values of threshold Ct cycles, on the basis of its changes in the dynamics of treatment, a decrease, stabilization or growth of the bacterial load is judged, where an increase in the ratio of the obtained values of threshold Ct cycles during therapy, an increase in the bacterial load is stated, while remaining unchanged: stabilization of the state, with a decrease a reduction in the bacterial load, which makes it possible to evaluate the effectiveness of leprosy therapy, inversely proportional to the bacterial load.

EFFECT: invention provides an assessment of the effectiveness of leprosy chemotherapy.

1 cl, 5 dwg, 1 tbl, 2 ex