FIELD: medicine.
SUBSTANCE: proposed inventions group relates to the field of medicine, in particular to immunology, pathophysiology and molecular biology. The proposed method and kit for determining the level of expression of the gene encoding the PEDF rabbit Oryctolagus cuniculus by PCR in real time.
EFFECT: proposed inventions group provides for obtaining a protocol for sample preparation, setting and calculating the results of RT-PCR with an optimally selected set of reagents that allow high accuracy to reproduce the reaction under the conditions of PCR laboratories for further use in experimental ophthalmology, in preclinical studies of medical devices and, in particular , to assess the state of the barrier systems of the eye and the effect on them of drugs developed for the treatment of eye diseases.
2 cl, 2 tbl, 1 ex, 2 dwg
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