IMPROVED PHARMACOKINETIC ASSAYS FOR SINGLE VARIABLE IMMUNOGLOBULIN DOMAINS Russian patent published in 2022 - IPC G01N33/53 G01N33/68 

FIELD: chemistry.

SUBSTANCE: invention relates to improved pharmacokinetic assays. Disclosed is a method for determining the amount and/or concentration of at least one immunoglobulin single variable domain (ISVD), or protein or polypeptide, including at least one ISVD, in a sample, including the following stages: a) contacting the sample with a capturing agent so that the ISVD, protein, or polypeptide is captured by the capturing agent; b) contacting the ISVD, protein, or polypeptide captured by the capturing agent with a detecting agent so that said detecting agent the agent binds to the ISVD, protein, or polypeptide captured by the capturing agent; c) generating a signal corresponding to the amount of the detecting agent bound to the ISVD, protein, or polypeptide captured by the capturing agent, in the presence of a quencher, wherein said quencher constitutes a protein or polypeptide including a VTVSS sequence (SEQ ID NO: 1) at the C-terminus thereof; binding to the monoclonal antibody 21-4 produced by the hybridoma ABH0015 deposited at BCCM, Gent, Belgium, under number LMBP-9680-CB, with an affinity equal to or greater than 1 mcM; binding to the capturing agent and the detecting agent with an affinity less than 3 mcm.

EFFECT: invention solves the problem of possible protein interference by performing an assay in the presence of a quencher whereto any pre-existing antibodies present in the sample can bind.

9 cl, 8 dwg, 2 tbl, 5 ex