BISPECIFIC BINDING PROTEINS AND APPLICATION WAYS THEREOF Russian patent published in 2022 - IPC A61K39/395 C07K16/28 C12N15/63 C12N5/10 A61P35/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology and immuno-oncology, in particular, to a bispecific binding protein binding to PD-1 and CTLA-4. The protein comprises a first heavy chain including CDR1 - GFTFSDYGMH, CDR2 - YISSGSYTIYSADSVKG, CDR3 - RAPNSFYEYYFDY; a first light chain including CDR1 - SASSKHTNLYWSRHMYWY, CDR2 - LTSNRAT, CDR3 - QQWSSNP; a second heavy chain including CDR1 - GFTFSSYGMH, CDR2 - VIWYDGSNKYYADSVKG, CDR3 - DPRGATLYYYYYGMDV; and a second light chain including CDR1 - RASQSINSYLD, CDR2 - AASSLQS, CDR3 - QQYYSTP. Also proposed are a composition containing such a protein, a nucleic acid molecule encoding the protein, a host cell for expression thereof, a method for treating or preventing cancer in a subject, and a method for increasing the immune response in a subject with cancer or subjected to a risk of developing cancer.

EFFECT: invention allows for production of bispecific binding proteins with improved binding properties relative to the initial monospecific binding proteins targeting the same molecules, even if used in combination.

12 cl, 90 dwg, 22 tbl, 3 ex