FIELD: biotechnology.
SUBSTANCE: invention relates to antitumor tetraspecific antibodies, and can be used in medicine for the treatment of a subject with a CD19-positive malignant neoplasm. The following was constructed: a recombinant tetraspecific antibody with specificity for binding to CD3, CD19, PD-L1 and 4-1BB containing the following in tandem from the N-terminus to the C-terminus: a binding domain for CD-3, a binding domain for CD19, an IgG Fc domain, a binding domain for PD-L1, and a binding domain for 4-1BB, wherein the binding domain for tumor-associated antigen is not adjacent to the binding domain for T-cell costimulatory receptor.
EFFECT: invention provides an antitumor agent having activity in redirecting PBMC T-cell activity and stimulating the proliferation of CD8 T-cells against an acute B lymphoblastic leukemia cell line.
5 cl, 23 dwg, 11 tbl, 21 ex
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