Show metadata Hide metadata

(19)

(11)

2 771 288

(13)

(51)

IPC

C12N5/16(2006-01-01)

A61K39/395(2006-01-01)

A61K39/42(2006-01-01)

(21) (22)

Application

2021102351, 2021-02-02

(24)

Start date

2021-02-02

(22)

Actual filing date

2021-02-02

(45)

Published

2022-04-29

(72)

Inventor

Cherepushkin Stanislav AndreevichGrebennikova Tatyana VladimirovnaTsibezov Valerij VladimirovichLarichev Viktor FilippovichShcheblyakov Dmitrij ViktorovichEsmagambetov Ilyas BulatovichFavorskaya Irina AlekseevnaDerkaev Artem AlekseevichGintsburg Aleksandr Leonidovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Issledovatelskij Tsentr Epidemiologii I Mikrobiologii Imeni Pochetnogo Akademika N.F. Gamalei" Ministerstva Zdravookhraneniya Rossijskoj Federatsii

STRAIN OF HYBRID ANIMAL CELLS MUS MUSCULUS 2E1B5 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE RECEPROT-BINDING DOMAIN OF PROTEIN S OF THE SARS-CoV-2 VIRUS Russian patent published in 2022 - IPC C12N5/16 A61K39/395 A61K39/42

Abstract RU 2771288 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular, to a strain of hybrid cultured animal cells Mus musculus 2E1B5. Hybrid clone of 2E1B5 cells was produced by fusion of mouse myeloma cells of the Sp2/0-Ag-14 line with splenocytes of mice of the Balb/c line, immunised intramuscularly with the recombinant receptor-biniding domain of protein S of the SARS-CoV-2 virus. Hybridoma 2E1B5 synthesises an IgG1-class monoclonal antibody specifically interacting with the recombinant receptor-binding domain of protein S of the SARS-CoV-2 virus, full-length S protein of the SARS-CoV-2 virus, and inactivated SARS-CoV-2 virus. The antibody exhibits virus-neutralising activity and is capable of neutralising the SARS-CoV-2 virus in vitro. The resulting antibody can be used in diagnostic studies in order to detect an antigen and specific antiviral antibodies and to create medicinal products against the SARS-CoV-2 virus.

EFFECT: invention expands the range of means for producing monoclonal antibodies against protein S of the SARS-CoV-2 virus.

1 cl, 1 dwg, 1 tbl, 5 ex

Similar patents RU2771288C1

Title	Year	Author	Number
STRAIN OF HYBRID ANIMAL CELLS Mus MUSCULUS 1F1 - PRODUCER OF A MONOCLONAL ANTIBODY AGAINST THE NUCLEOCAPSID PROTEIN N OF THE SARS-CoV-2 VIRUS	2021	Cherepushkin Stanislav Andreevich Grebennikova Tatyana Vladimirovna Tsibezov Valerij Vladimirovich Larichev Viktor Filippovich Shcheblyakov Dmitrij Viktorovich Esmagambetov Ilyas Bulatovich Favorskaya Irina Alekseevna Derkaev Artem Alekseevich Gintsburg Aleksandr Leonidovich	RU2769817C1
MONOCLONAL ANTIBODY TO RDB FRAGMENT IN COMPOSITION OF SARS-COV-2 S PROTEIN	2020	Shakhparonov Mikhail Ivanovich Pavliukov Marat Samvelovich Antipova Nadezhda Viktorovna	RU2744274C1
HUMANIZED MONOCLONAL ANTIBODY SPECIFICALLY BINDING TO RBD S OF THE PROTEIN OF THE SARS-COV-2 VIRUS, AGENT AND METHOD FOR THERAPY AND EMERGENCY PREVENTION OF DISEASES CAUSED BY SARS-COV-2 VIRUS	2021	Esmagambetov Ilias Bulatovich Shchebliakov Dmitrii Viktorovich Lebedin Iurii Stepanovich Favorskaia Irina Alekseevna Dolzhikova Inna Vadimovna Derkaev Artem Alekseevich Riabova Ekaterina Igorevna Prokofev Vladimir Vladimirovich Alekseeva Irina Aleksandrovna Voronina Daria Vladimirovna Zorkov Ilia Dmitrievich Kovyrshina Anna Vitalevna Iliukhina Anna Alekseevna Botikov Andrei Gennadevich Karpov Andrei Pavlovich Lubenets Nadezhda Leonidovna Zubkova Olga Vadimovna Semikhin Aleksandr Sergeevich Naroditskii Boris Savelevich Logunov Denis Iurevich Gintsburg Aleksandr Leonidovich	RU2765731C1
MEANS AND METHOD FOR THERAPY AND EMERGENCY PREVENTION OF DISEASES CAUSED BY THE SARS-COV-2 VIRUS BASED ON A RECOMBINANT ANTIBODY AND A HUMANIZED MONOCLONAL ANTIBODY	2021	Shchebliakov Dmitrii Viktorovich Esmagambetov Ilias Bulatovich Favorskaia Irina Alekseevna Dolzhikova Inna Vadimovna Lebedin Iurii Stepanovich Derkaev Artem Alekseevich Riabova Ekaterina Igorevna Prokofev Vladimir Vladimirovich Alekseeva Irina Aleksandrovna Voronina Daria Vladimirovna Zorkov Ilia Dmitrievich Kovyrshina Anna Vitalevna Iliukhina Anna Alekseevna Botikov Andrei Gennadevich Karpov Andrei Pavlovich Lubenets Nadezhda Leonidovna Zubkova Olga Vadimovna Semikhin Aleksandr Sergeevich Naroditskii Boris Savelevich Logunov Denis Iurevich Gintsburg Aleksandr Leonidovich	RU2769223C1
THE STRAIN OF HYBRID CULTURED HOMO SAPIENS/Mus MUSCULUS CELLS Hu-C6D7-RBD IS A PRODUCER OF HUMAN MONOCLONAL ANTIBODIES SPECIFIC TO THE RBD DOMAIN S OF THE SARS-CoV-2 VIRUS PROTEIN	2022	Romanenko Iana Olegovna Silkina Marina Vladimirovna Kartseva Alena Sergeevna Marin Maksim Aleksandrovich Konyshkova Daria Andreevna Makarova Mariia Aleksandrovna Shkuratova Mariia Andreevna Shemiakin Igor Georgievich Firstova Viktoriia Valerevna	RU2788359C1
POLYPEPTIDE WITH ANTI-SARS-COV-2 ACTIVITY AND PROLONGED CIRCULATION TIME IN BLOOD FLOW	2023	Bobik Tatyana Vladimirovna Simonova Mariya Aleksandrovna Rushkevich Natalya Yurevna Kostin Nikita Nikolaevich Skryabin Georgij Andreevich Shulga Aleksej Anatolevich Konovalova Elena Valerevna Lukyanova Tamara Ivanovna Ziganshin Rustam Khusmanovich Proshkina Galina Mikhajlovna Gabibov Aleksandr Gabibovich Deev Sergej Mikhajlovich	RU2822355C1
SINGLE-DOMAIN LLAMA ANTIBODY H5 AND ITS DERIVATIVE H5-Fc SPECIFICALLY BINDING THE RBD DOMAIN OF THE S-PROTEIN OF THE SARS-CoV-2 VIRUS POSSESSING VIRUS-NEUTRALIZING ACTIVITY	2022	Baranov Konstantin Olegovich Belovezhets Tatyana Nikolaevna Volkova Olga Yurevna Gorchakov Andrej Aleksandrovich Guselnikov Sergej Vladimirovich Kulemzin Sergej Viktorovich Mechetina Lyudmila Vasilevna Nayakshin Aleksandr Matveevich Solodkov Pavel Pavlovich Taranin Aleksandr Vladimirovich Chikaev Anton Nikolaevich Chikaev Nikolaj Andreevich	RU2793967C1
BROADLY NEUTRALIZING ANTIBODY AGAINST SARS-CoV-2	2022	Mokrushina Iuliana Anatolevna Terekhov Stanislav Sergeevich Malabuiok Diana Maksiminovna Ovchinnikova Leila Aleksandrovna Abrikosova Viktoriia Aleksandrovna Lomakin Iakov Anatolevich Baranova Margarita Nikolaevna Kostin Nikita Nikolaevich Kalinin Roman Sergeevich Bobik Tatiana Vladimirovna Smirnov Ivan Vitalevich Gabibov Aleksandr Gabibovich	RU2810476C1
MONOCLONAL ANTIBODY IC1 AND ITS ANTIGEN-BINDING FRAGMENT, SELECTIVELY BINDING RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 VIRUS, HAVING VIRUS-NEUTRALIZING ACTIVITY	2023	Baranov Konstantin Olegovich Belovezhets Tatyana Nikolaevna Volkova Olga Yurevna Gorchakov Andrej Aleksandrovich Guselnikov Sergej Vladimirovich Kulemzin Sergej Viktorovich Mechetina Lyudmila Vasilevna Nayakshin Aleksandr Matveevich Solodkov Pavel Pavlovich Taranin Aleksandr Vladimirovich Chikaev Anton Nikolaevich Chikaev Nikolaj Andreevich	RU2817696C1
MONOCLONAL ANTIBODY IC2 AND ITS ANTIGEN-BINDING FRAGMENT, SELECTIVELY BINDING RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 VIRUS, HAVING VIRUS-NEUTRALIZING ACTIVITY	2023	Baranov Konstantin Olegovich Belovezhets Tatyana Nikolaevna Volkova Olga Yurevna Gorchakov Andrej Aleksandrovich Guselnikov Sergej Vladimirovich Kulemzin Sergej Viktorovich Mechetina Lyudmila Vasilevna Nayakshin Aleksandr Matveevich Solodkov Pavel Pavlovich Taranin Aleksandr Vladimirovich Chikaev Anton Nikolaevich Chikaev Nikolaj Andreevich	RU2817697C1

RU 2 771 288 C1

Authors

Cherepushkin Stanislav Andreevich

Grebennikova Tatyana Vladimirovna

Tsibezov Valerij Vladimirovich

Larichev Viktor Filippovich

Shcheblyakov Dmitrij Viktorovich

Esmagambetov Ilyas Bulatovich

Favorskaya Irina Alekseevna

Derkaev Artem Alekseevich

Gintsburg Aleksandr Leonidovich

Dates

2022-04-29—Published

2021-02-02—Filed