EXOGENOUS CONTROL OF GENE EXPRESSION OF MAMMALS USING APTAMER-MEDIATED POLYADENYLATION MODULATION Russian patent published in 2022 - IPC C12Q1/68 C12N15/09 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to the use of expression structures, in which at least one polyA signal is placed on the left in the course of transcription of an expressed transcript, for example, within 5' untranslated region (hereinafter – UR) transcript. In some embodiments, the polyA signal is contained within ligand-binding aptamer, and binding of ligand with aptamer or absence of binding determines the release of the expressed transcript. In specific embodiments, the absence of ligand causes the expression of the expressed transcript containing polyA in its 5' UR, but then its destruction occurs, whereas the presence of ligand causes the inhibition of destruction in the expression of the expressed transcript. There can be more than one ligand-binding aptamer on the same expression structure.

EFFECT: obtaining expression structures.

64 cl, 31 dwg, 1 tbl, 3 ex