FIELD: biotechnology.
SUBSTANCE: mutation (mutations) or modification (modifications) of CRISPR enzyme, for example, Cas enzyme, such as Cas9, are disclosed and claimed. Methods for producing and applying and options of applying such a mutated or modified Cas, or CRISPR enzyme, or Cas9, and systems or complexes containing the above mentioned and products obtained in the result of such methods are also disclosed and claimed.
EFFECT: invention allows reducing non-targeted effects of CRISPR-Cas enzyme, or CRISPR enzyme, or system or complex of CRISPR-Cas9 containing or including such a mutated or modified Cas, or CRISPR or Cas9 enzyme.
81 cl, 23 tbl, 19 ex, 81 dwg
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