FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, particularly to regulation of gene expression. Invention discloses a genetic construct containing a polyA aptamer polynucleotide, which contains in direction from 5' to 3': a) 5'-donor splice site; b) engineered intron; c) first 3'-acceptor splice site; d) a polyA switch containing two or more ligand-binding aptamers with one or more ligand-binding pockets, and at least one polyA cleavage signal therein; e) second 3'-acceptor splice site; and f) a nucleic acid sequence encoding the expressed polypeptide.
EFFECT: construct according to the present invention is able to significantly enhance the expression of the target gene and can be used in the production of protein preparations.
45 cl, 23 dwg, 1 tbl, 15 ex
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