FIELD: medicine; immunology.
SUBSTANCE: group of inventions relates to medicine and immunology, specifically to the use of compositions containing mutant toxins Clostridium difficile. The use of a composition containing purified mutant toxin A C. difficile containing an amino acid sequence represented in SEQ ID NO: 4 is proposed, where methionine residue in a position 1 is probably absent, for inducing an immune response against toxin A C. difficile in a mammal, where the composition is intended for the injection to the specified mammal three times during four weeks. Options of the use of a composition containing purified cross-linked mutant toxin A C. difficile are also proposed, where cross-linked toxin A C. difficile is obtained by bringing into contact with a cross-linking agent selected from formaldehyde, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) and EDC/NHS combination, and where toxin A C. difficile is obtained by purifying toxin A C. difficile by means of chromatography with hydrophobic interaction with subsequent bringing into contact with formaldehyde, while the specified composition additionally contains 0.001-0.010% of formaldehyde.
EFFECT: group of inventions allows for obtaining immunogenic compositions aimed at C. Difficile.
42 cl, 25 dwg, 61 tbl, 45 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMPOSITIONS RELATED TO MUTANT TOXIN CLOSTRIDIUM DIFFICILE AND THEIR APPLICATION METHODS | 2012 |
|
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| FUSED MOLECULES DERIVED FROM CHOLIX-TOXIN FOR ORAL DELIVERY OF BIOLOGICALLY ACTIVE LOADS | 2015 |
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| MUTANT NITRILE HYDRATASE, NUCLEIC ACID WHICH ENCODES SAID MUTANT NITRILE HYDRATASE, EXPRESSION VECTOR AND TRANSFORMANT CONTAINING SAID NUCLEIC ACID, METHOD OF PRODUCING SAID MUTANT NITRILE HYDRATASE AND METHOD OF PRODUCING AMIDE COMPOUND | 2017 |
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| RSV F MUTANT PROTEIN AND ITS APPLICATION | 2020 |
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| FUSED SERPINE POLYPEPTIDES AND METHODS OF THEIR APPLICATION | 2015 |
|
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| MOLECULES TARGETING A TYPE III SECRETION SYSTEM | 2016 |
|
RU2759949C2 |
| BISPECIFIC ANTIBODY AGAINST RABIES VIRUS AND ITS APPLICATION | 2019 |
|
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| TETANUS TOXIN ANTIBODY AND USE THEREOF | 2020 |
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| HERPES SIMPLEX SYNCYTIAL ONCOLYTIC MUTANTS AS POWERFUL THERAPEUTIC AGENTS FOR TREATING CANCER | 2020 |
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RU2821999C2 |
| POLYVALENT AND POLYSPECIFIC GITR-BINDING FUSED PROTEINS | 2016 |
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