FIELD: biotechnology; medicine.
SUBSTANCE: invention relates to biotechnology and medicine and is a constructed half-domain of FokI cleavage. The invention also relates to a constructed nuclease containing the specified cleavage domain, as well as is a method for cleavage of genomic cellular chromatin in an area of interest, including expression of an artificial nuclease containing the constructed domain of cleavage in a cell, wherein the nuclease site-specifically cleaves a sequence of nucleotides in the area of interest of genomic cellular chromatin.
EFFECT: invention provides for obtaining an effective cleavage system for reduction in non-target cleavage activity.
17 cl, 18 dwg, 18 tbl, 9 ex
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