FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Methods are proposed for introducing a seamless targeted genetic modification into a previously created directed vector.
EFFECT: invention can be used to simplify the creation of a targeting that carries multiple changes (such as a humanized allele and a layered disease mutation) and to minimize the seams involved in the final animal model.
30 cl, 5 dwg, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MICE CONTAINING MUTATIONS, AS A RESULT OF WHICH FIBRILLIN-1 SHORTENED AT C-TERMINUS IS EXPRESSED | 2017 |
|
RU2721125C1 |
| METHODS AND COMPOSITIONS FOR ASSESSMENT OF MEDIATED CRISPR/CAS DESTRUCTION OR CUTOUT AND INDUCED CRISPR/CAS RECOMBINATION WITH EXOGENEOUS DONOR NUCLEIC ACID IN VIVO | 2018 |
|
RU2782356C2 |
| ANIMALS OTHER THAN HUMAN, INCLUDING HUMANIZED TTR LOCUS, AND APPLICATION METHODS | 2018 |
|
RU2784927C1 |
| NON-HUMAN ANIMALS CONTAINING HUMANISED ALBUMIN LOCUS | 2020 |
|
RU2815514C2 |
| CAS-EXPRESSING MOUSE EMBRYONIC STEM CELLS, AND MICE AND THEIR USE | 2018 |
|
RU2782358C2 |
| CRISPR/CAS9 TYPE II GENOME EDITING SYSTEM AND ITS USE | 2022 |
|
RU2794774C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM DEMEQUINA SEDIMINICOLA BACTERIA | 2019 |
|
RU2722933C1 |
| USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS | 2019 |
|
RU2724470C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
|
RU2722934C1 |
| GENE EDITING OF DEEP INTRON MUTATIONS | 2016 |
|
RU2759335C2 |
