METHOD FOR CULTIVATION OF BACKSHOT CLADOCER MOINA SALINA Russian patent published in 2022 - IPC A01K61/20 

FIELD: marine aquaculture.

SUBSTANCE: method relates to the field of marine aquaculture and is intended to obtain a mass of high-quality live food organisms for use as live food in the cultivation of valuable marine fish larvae and for experimental work in the field of scientific research in biology, embryology, genetics, physiology, biochemistry and biological testing. Female moins taken from a natural zooplankton sample are kept in a disinfectant solution with the addition of microalgae for 6-8 hours, placed in containers with sterile sea water with a minimum volume of 30 ml per female, and a constant illumination of 5000 lux, microalgae are fed. Then the resulting progeny is used for cumulative cultivation at the parthenogenetic stage of reproduction, which is carried out at a temperature of 24±2°C, illumination on the cultivator surface of 5000 lux, a photoperiod of at least 16 hours of illumination and 8 hours of darkness, with a consistent increase in the density of females up to 300 ind/ l, in a non-removable medium, with daily application of microalgae. After that, while maintaining a constant density of females of about 300 ind/l, the culture volume is increased, with the daily addition of sterile sea water with microalgae, after which moins are cultivated with a daily withdrawal of about 0.25 of the culture volume, replacing this volume with sterile sea water and introducing microalgae, which are used as Isochrysis galbana, Tetraselmis suecica and Rhodomonas sp., maintaining their concentration during the parthenogenetic stage of reproduction from 3 to 10 mcg/ml. Upon completion of the increase in the biomass of parthenogenetic female moins, they are transferred to the stage of sexual reproduction, increasing the culture density to 3000-5000 ind/l and keeping at this density for 48 hours, reducing the water temperature during this time from 25°C to 15°C, with illumination on the surface cultivator 500 lx, photoperiod 5-6 hours of light and 18-19 hours of darkness and concentration of microalgae 1-2 mcg/l. Ephippiums obtained as a result of sexual reproduction are dried and stored for 6-24 months. To transfer the resting embryos of moins into an active state and obtain juvenile parthenogenetic females of moins, ephippiums are placed at a density of about 300 ind/l for 24-48 hours in sterile cold sea water with a salinity of 18, washed several times and gradually over 12 hours raise the water temperature to 25°C, simultaneously increasing the oxygen level to 85-100% saturation, with constant illumination on the surface of the incubator of at least 1000 lux.

EFFECT: invention ensures the production of mass high-quality live feed of a homogeneous composition with a high content of protein and essential amino acids.

1 cl, 4 dwg, 1 ex