METHOD FOR CULTIVATION OF OITHONA DAVISAE MARINE CYCLOPOID COPEPODS Russian patent published in 2023 - IPC A01K61/20 

FIELD: marine aquaculture.

SUBSTANCE: method for cultivation of Oithona davisae marine cyclopoid copepods is claimed, including obtainment of initial adult oithon specimens from plankton samples selected from the sea, disinfection, and adaptation to cultivation conditions. Then, mature oithon females are contained for 4 days in a cultivator with sterilized seawater with a density of 0.1 pieces/ml, with daily feeding with nano-plankton microalgae in a concentration of 10⁴-10⁵ c/ml. 12 hours after mass hatching, nauplii are separated from females and cultivated for 12 days in sterile seawater, gradually reducing a copepod density from 5 pieces/ml to 0.1 pieces/ml until reaching maturity of the same-age cohort, with daily correction of the concentration of microalgae to a level of 5×10⁴ c/ml. Then, fertilization of females by males is stimulated during 2 days, reducing a reached cultivation volume by reduction in a temperature to 15°C, daylight to 5 h, and concentration of microalgae to 10³ c/ml, then, a temperature is reduced to 10°C. Females in containers with sterile seawater, with a small concentration of food, are placed in a refrigeration installation at 7±1°C, in complete darkness, where they are contained for 2-4 months with weekly purification of containers, replacement of 50% of the medium, with addition of 10³ c/ml of microalgae. 4 days before the beginning of transition to external feeding of cultivated larvae of marine fish, in hibernating oithon females, a temperature is increased to 17°C, washing with a sterile solution, they are fed with a mixture of microalgae with addition of heterotrophic Oxyrrhls dinoflagellate and transferred to containers for larvae, to which microalgae is continued to be added.

EFFECT: invention provides obtainment of a feed product of a corresponding size for transition of fish larvae with pelagic caviar to external feeding.

1 cl, 5 dwg, 1 tbl, 1 ex