METHOD FOR CULTIVATION OF MARINE HETEROTROPHIC DINOFLAGELLATES OXYRRHIS MARINA Russian patent published in 2023 - IPC A01G33/00 

FIELD: marine aquaculture.

SUBSTANCE: intended to obtain a mass of high-quality live food organisms for use as intermediate live food for marine copepods, for experimental work, and also as a source of valuable chemical compounds such as highly unsaturated fatty acids and sterols. Oxyrrhis marina cells are isolated from natural reservoirs and adapted to cultivation conditions. During the adaptation process, the fastest growing cells are selected during cultivation for 7-14 days using sterile sea water, at an initial density of O. marina 0.5 cells/ml, with feeding once for 3 days with nanoplanktonic microalgae at a concentration of ~10⁴ cells/ml and constant illumination of 500 lux, and then the culture of O. marina is recultivated under conditions of reduced metabolism at 15°C with minimal lighting~ 100-500 lux, when feeding 1-2 times a month with a suspension of ~10⁴ cells/ml of green microalgae Tetraselmis or Dunaliella. To obtain a mass culture of heterotrophic dinoflagellates O. marina, they are transferred to conditions for activating cell metabolism and stimulating accelerated division by increasing the temperature to the optimal 24±1°C and optimization of feeding microalgae Isochrysis galbana 10⁵ cells/ml and for 3 days with a gradual increase in the volume of dinoflagellate culture from 20 to 200 ml at a cell density of O. marina ~300 cells/ml and an average population growth rate of 1.4 days^-1, then, over the course of 5 days at a population growth rate of 0.7-1.4 day^-1, a gradual increase in cell density is made from 300 to ~40,000 cells/ml while simultaneously increasing the volume to 1000 ml by adding a nutrient suspension of I. galbana cells in sterile sea water. To transfer the resulting stock culture to a semi-industrial one, 0.5 volumes of the intensive culture of O. marina are removed every day, replenishing the withdrawal to the full volume with a suspension of sterile sea water with the addition of a mixture of microalgae Tetraselmis, Rhodomonas, Phaeodactylum, and after a day the removal procedure is repeated.

EFFECT: possibility of obtaining Oxyrrhis marina biomass.

1 cl, 4 dwg, 1 tbl, 1 ex